MersV1, Main, Exploration, bibRecord, 004D58

Analysis of Expression of Yeast Enolase 1 Gene Containing a Longer Pyrimidine-Rich Region Located between the TATA Box and Transcription Start Site

Identifieur interne : 004D58 ( Main/Exploration ); précédent : 004D57; suivant : 004D59

Analysis of Expression of Yeast Enolase 1 Gene Containing a Longer Pyrimidine-Rich Region Located between the TATA Box and Transcription Start Site

Auteurs : Yoshifumi Jigami ; Nobumasa Toshimitsu ; Hirofumi Fujisawa ; Hiroshi Uemura ; Hideaki Tanaka ; Satoshi Nakasato

Source :

The Journal of Biochemistry [ 0021-924X ] ; 1986.

RBID : ISTEX:37E1EF542B8B2FD075841BFA33EC187EFD50CD16

Abstract

Yeast ENO1 promoter was prepared by a chemical synthetic method. Two variant promoters containing a pyrimidine-rich region (CT block), located between the TATA box and the transcription start site, either 32 or 34 base pairs (bp) longer than the native ENO1 promoter were isolated during the chemical synthesis. Gene expression of variant promoters was compared with that of the native promoter by measuring the amount of mRNA and the activity of β-galactosidase by constructing ENO1-lacZ gene fusions. No significant differences were observed between the native and variant promoters in transcription levels. The start site of transcription was mapped on CAAG, a consensus sequence of transcription start site of yeast glycolytic genes. The results suggest a longer CT block in ENO1 promoter may not affect the expression of the yeast ENO1 gene. In addition, the level of ENO1 gene expression was found to be higher in stationary phase cells than in log phase cells.

Url:

https://api.istex.fr/ark:/67375/HXZ-VW8P6DZX-R/fulltext.pdf

DOI: 10.1093/oxfordjournals.jbchem.a135575

Affiliations:

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Le document en format XML

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<front><div type="abstract">Yeast ENO1 promoter was prepared by a chemical synthetic method. Two variant promoters containing a pyrimidine-rich region (CT block), located between the TATA box and the transcription start site, either 32 or 34 base pairs (bp) longer than the native ENO1 promoter were isolated during the chemical synthesis. Gene expression of variant promoters was compared with that of the native promoter by measuring the amount of mRNA and the activity of β-galactosidase by constructing ENO1-lacZ gene fusions. No significant differences were observed between the native and variant promoters in transcription levels. The start site of transcription was mapped on CAAG, a consensus sequence of transcription start site of yeast glycolytic genes. The results suggest a longer CT block in ENO1 promoter may not affect the expression of the yeast ENO1 gene. In addition, the level of ENO1 gene expression was found to be higher in stationary phase cells than in log phase cells.</div>
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Analysis of Expression of Yeast Enolase 1 Gene Containing a Longer Pyrimidine-Rich Region Located between the TATA Box and Transcription Start Site

Analysis of Expression of Yeast Enolase 1 Gene Containing a Longer Pyrimidine-Rich Region Located between the TATA Box and Transcription Start Site

Source :

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri